Echinoderms, Volume 150 in the Methods in Cell Biology series, highlights new advances in the field, with this update presenting interesting chapters on procuring animals and culturing of eggs and embryos, cryopreservation of sea urchin gametes, emerging echinoderm models, culturing of sand dollars, cidaroids and heart urchins, culturing echinoderm larvae through metamorphosis, microinjection methods, injection of exogenous messages and protein overexpression, blastomere transplantation, visualization of embryonic polarity, larval immune cell approaches, methods for analysis of sea urchin primordial germ cells, and protocols and best practices for toxicology and pH studies using echinoderms and several new chapters outlining the use of sea urchins in the classroom.